Cell kinetic and histomorphometric analysis of microgravitational osteopenia Download PDF EPUB FB2
Get this from a library. Cell kinetic and histomorphometric analysis of microgravitational osteopenia: PAREB: final report: NAG [W Eugene Roberts; Lawrence P Garetto; United States. National Aeronautics and Space Administration.]. Cell Kinetic and Histomorphometric Analysis of Microgravitational Osteopenia: PAREB.
By Lawrence P. Garetto and W. Eugene Roberts. Abstract. Previous methods of identifying cells undergoing DNA synthesis (S-phase) utilized H-3 thymidine (3HT) autoradiography.
5-Bromo-2'-deoxyuridine (BrdU) immunohistochemistry is a nonradioactive Author: Lawrence P. Garetto and W. Eugene Roberts. Cell Kinetic and Histomorphometric Analysis of Microgravitational Osteopenia: PAREB. By W. Eugene Roberts and Lawrence P. Garetto. Abstract. The final report includes the hypotheses and specific aims of the project as well as summaries of the experimental findings.
Descriptions of several figures that are not included in the report are also Author: W. Eugene Roberts and Lawrence P. Garetto. A large number of histomorphometric variables can be measured or derived.
Because the morphometric analysis is extremely time-consuming, the number of variables evaluated depends on whether the biopsy specimen is being analyzed for diagnostic or research purposes. Listed next are eight indices of trabecular bone that are of particular clinical Cited by: 4.
Lawrence P. Garetto's 35 research works with 1, citations and 2, reads, including: Decreased alveolar bone turnover is related to the occurrence of root resorption during experimental tooth. However, the histomorphometric indices that describe the mechanisms of bone loss are important in the understanding of the usefulness and limitations of the histomorphometric method.
Using osteoporosis as an example, this chapter presents the methodological aspects of. Histomorphometry is the method used to quantify histological features of bone (quantitative bone histology) 8,10,15,30–32, in particular static and dynamic parameters of bone structure, formation and main use is in clinical studies in which reproducibility is requi especially for the evaluation of the effects of a given treatment on resorptive and formative bone cells Histomorphometric Analysis.
Histomorphometric analysis of bone can range from simple assessments of bone structure to more detailed analyses of cell numbers and function.
An essential reference for anyone interested in histomorphometry is the publication by A. Michael Parfitt and colleagues. Bone is a dynamic tissue that undergoes constant remodeling, balancing bone resorption by osteoclasts with bone formation by osteoblasts (Baron, ; Karsenty and Wagner, ).An altered balance between bone formation and bone resorption causes diseases such as osteoporosis and osteopetrosis (Seeman, ; Villa et al., ).It is well known that osteoclasts are responsible for.
Cell Metabolism Cell Homeostasis and Stress Response 16 The analysis of differential gene expression and analysis of global patterns of gene expression using macro and microarray approaches have identified a broad spectrum of transcripts whose expression is modified in response to dehydration (Fowler & Thomashow, ; Kreps et al., ; Seki.
Toggle navigation Life Sciences Data Archive. HOME ; FOR RESEARCHERS ; FOR EDUCATORS; FOR STUDENTS; FOR EVERYONE; Search By: Experiment; Mission ; Personnel. Remodeling is the physiologic term for internal turnover of a mineralized tissue, without a change in its overall form.
It is a coupled sequence of catabolic (resorptive) and anabolic (osteogenic) events to support calcium homeostasis and repair (renew) aged or damaged mineralized tissue.
Histological studies indicate that both bones and teeth remodel. To perform kinetic analyses of bone formation and mineralization, calcein was injected twice at a 3-d interval in 3-wk-old mice and a 7-d interval in 6-wk-old mice.
Calcein injections formed two consecutive labels in most parts of 3- and 6-wk-old wild-type bone (Fig. 7 A). Author(s): Roberts,W Eugene; Garetto,Lawrence P; IUPUI (Campus) Investigator(s): Roberts,W Eugene; Non-NASA Center: IN U, Indianapolis.
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Nanobiomaterials in Hard Tissue Engineering covers the latest developments in the field of hard tissue engineering at the g researchers from around the world discuss the latest research and offer new insights.
This book presents data about the fabrication and characterization of nanobiomaterials involved in hard tissue reconstruction, describing recent progress and the. The study was prompted by the rapid recovery of bone mass following severe osteopenia (decreased bone mass) caused by inducible osteoblast ablation, which suggested precise control of bone mass.
The histomorphometric analysis was done on a Leica quantimet image processor and the followings measures were performed: trabecular bone volume (BV/TV), trabecular thickness (Tb Th) and number (Tb N), Interconnectivity Index (ICI), Star Volume of the bone marrow, Characterization of the trabecular network (node and free-end count).
Histomorphometric analysis. At yr, a left iliac crest biopsy was performed as described in the Patients and Methods. The static histomorphometric data are listed in Table 1 Most striking is thinner than normal cortical bone, similar to osteoporotic females, and attenuated trabecular thickness, despite normal trabecular number and spacing.
In our previous investigation, delphinidin, one of the most abundant anthocyanins found in vegetables and berry fruits, had been shown to inhibit osteoclasts and prevent bone loss in mouse models of osteoporosis. In the present study, we investigated whether a delphinidin glycoside-enriched maqui berry extract (MBE, Delphinol®) exhibits beneficial effects on bone metabolism both in vitro and.
The histomorphometric analysis indicated that both the amount of bone formed during 30 days and the mineral apposition rate during 10 days (deduced from the interval between the two tetracycline labels) were significantly greater (p.
Journal of Law and Education Journal of Economic Education Books by Language Journal of materials engineering. Additional Collections Journal of Management Studies Journal of Autism and Developmental Disorders Featured. Although, based on the kinetic analysis, the growth rates of BMMSC during the exponential growth phase were not significantly affected, % higher cell densities were achieved during the exponentially expanding stage.
Many newly divided cells appeared from 12 to 16 h after the PEMF treatment as revealed by the cell cycle analysis. Ben‐Ze'ev, A., Robinson, G.S., Bucher, N.L.R. and Farmer, S.R. () Cell‐cell and cell‐matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes.
Proceedings of the National Academy of Sciences of. A meta-analysis included six RCTs ( participants) of vitamin D 3 supplementation for 1–2 years' duration in children aged 8–17 years, The meta-analysis found supplementation had no effect on total-body BMC or BMD of the hip or forearm, although there was a trend to a small effect on lumbar spine density and towards a larger.
Histomorphometric analysis revealed a minimal effect on bone-formation parameters with the administration of low-dose anti-resorptives in Trial V.
For most treatment groups, no significant change in BFR, AMPm, or MAR was observed (P > ). Bone turnover comprises two processes: the removal of old bone (resorption) and the laying down of new bone (formation).
N-terminal propeptide of type I procollagen (PINP) and C-telopeptide of type I collagen (CTX-I) are markers of bone formation and resorption, respectively, that are recommended for clinical use. Bone turnover markers can be measured on several occasions in one individual.
periodontology - Free ebook download as PDF File .pdf), Text File .txt) or read book online for free. perio. Free Online Library: Influence of bisphosphonates on alveolar bone density: a histomorphometric analysis.(Oral Pathology, Perspectiva general de la enfermedad/trastorno) by "Brazilian Oral Research"; Health, general Enfermedades dentales Analisis de casos Causas Cuidado y tratamiento Ratones como animales de laboratorio Historia Investigacion cientifica.
Cells were seeded at a density of 10 cells/cm 2 in well plates. Subsequently, the transwell supports containing the fibers were placed in the wells ensuring complete submersion of the fibers in the medium.
Cells were cultured for 1, 3, and 7 days (medium was replaced after 3 days) in an incubator with 5% CO 2 at 37 °C. 1. Introduction.
Bone is a mineralized connective tissue that exhibits four types of cells: osteoblasts, bone lining cells, osteocytes, and osteoclasts [1, 2].Bone exerts important functions in the body, such as locomotion, support and protection of soft tissues, calcium and phosphate storage, and harboring of bone marrow [3, 4].Despite its inert appearance, bone is a highly dynamic organ that.Effects of alfacalcidol and estrogen on biochemical markers of bone metabolism in OVX rats.
Ovariectomized rats (OVX, 8 months old) were given p.o. alfacalcidol (1α(OH)D 3:, and μg/kg body weight [BW]) or s.c. 17β‐estradiol (E 2: 4, 20, and μg/kg BW) five times a week for 3 months, and urinary deoxypyridinoline (D‐Pyr) excretion (A) and serum osteocalcin.Interestingly, mutational analysis on the tissues with heterogeneous p53 expression in the same patient showed that p53 mutation was present only in the p53 protein-positive cells exhibiting higher grade histology than p53 protein-negative, non-mutated cells.